We are hiring a Research Associate on a contract basis. This position is based near Boston, MA.
This research associate position will support the implementation and establishment of scalable viral vector manufacturing using mammalian cell line platforms. The individual in this position will be responsible for assisting with biological experiments semi-independently in accordance with manager approval in the area of AAV cell-line and process development for gene therapy production. Research experiments will encompass cell culture process development, media development, bioreactor scale-up, and execution of quantitative biological assays.
Responsibilities
- Actively contribute to programs to enhance AAV development and manufacturing technologies/ capabilities and work-processes (such as media development, feed development, bioreactor fed-batch and perfusion process development, optimization and scale-up)
- Works directly with supervision to design and execute experiments the bench for research, development, and evaluation of novel cell culture processes and scale-up of AAV technology platform
- Demonstrate ability to learn quickly- proper aseptic technique, new biological techniques (qPCR), and independent operation of lab scale cell culture equipment, metabolite and cell analyzers, and small-scale bioreactors
- Ensure high-quality, timely documentation in electronic laboratory notebooks and technical reports
- Collect and controls experimental data and maintains accurate daily logs of experiments including methods, results, and conclusions
- Assists in general laboratory maintenance duties
- Occasional weekend sampling/operation may be required, and this role will support weekend activities.
Requirements
- BS/MS in Chemical/Biochemical/Biomedical Engineering or related scientific discipline with 0-3 years academic/industry experience
- Strong math and science fundamentals are desired, in particular solution/dilution math, good understanding of molecular biology, and understanding of fluid mechanics/engineering principles as they relate to bioprocess and cell culture
- Experience with mammalian cell culture and aseptic technique in a BSL2 lab is preferred
- Experience with suspension culture, recombinant viruses, cell and molecular biology, qPCR, transient transfection are preferred
- Experience with bioreactors and downstream purification (are a plus)
- Ability and desire to work in a fast-paced environment
- Strong collaboration, team-working skills and communication skills
- Independently motivated and detail-oriented with good problem-solving ability
